Classic Spectrometer Analyzer Procedure

Spectrophotometry is an experimental technique that is used to measure the concentration of solutes in a specific solution by calculating the amount of light absorbed by those solutes.

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Classic Spectrometer Analyzer Procedure

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STANDARD OPERATING PROCEDURE Do not replace cuvette in rear holder. Using the same cuvette style, fill an empty cuvette with about 2-ml of the sample. Clean the cuvette with a lab tissue.

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Take at least 2-3 readings of your unknown and be sure to adjust the spectrophotometer to 0.0 for absorbance and 100 % for transmittance, before each reading. (This is to read out errors caused by

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