This article describes the principles of a high-precision calibration method that utilizes a Fabry-Perot multilayer structure, providing multiple sharp calibration peaks over the full spectrometer range. In most cases, spectrometers are calibrated using conventional. Methods for determining the accuracy of the emission (EM) or excitation (EX) wavelength for a fluorescence instrument are given here with an emphasis on monochromator (mono) based wavelength selection. An example spectrum, which has been spectrally corrected, is given for each method. Within the last decades, luminescence techniques developed into analytical tools. Agilent fluorescence calibration standards and kits provide an inexpensive and time-saving solution for calibrating and qualifying your fluorescence instrumentation. colicells, the stability of the protein and the high cost of its purification are problematic. We therefore use the small molecule fluorescein, which has. By following the general guidelines (Section 5) and instrument calibration methods (Sections 6-16) in this guide, users should be able to more easily conform to good laboratory and manufacturing practices (GXP) and comply with regulatory and QA/QC requirements, related to fluorescence measurements. 01 on Ultra-Violet, Visib e, and Luminescence Spec oved Nov.